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Scientists have mimicked an embryo’s heart to unlock the secrets of how blood cells are born

<p>Stem cells are the starting point for all other cells in our bodies. The <a href="https://www.eurostemcell.org/blood-stem-cells-pioneers-stem-cell-research" target="_blank" rel="noopener">first such cells to be found</a> were blood stem cells – as the name suggests, they give rise to different types of blood cells.</p> <p>But there’s much we don’t know about how these cells develop in the first place. In a study published today in <a href="https://doi.org/10.1016/j.celrep.2022.111339" target="_blank" rel="noopener">Cell Reports</a>, we have shown how a lab simulation of an embryo’s beating heart and circulation lead to the development of human blood stem cell precursors.</p> <p>The tiny device mimics embryonic blood flow, allowing us to directly observe human embryonic blood formation under the microscope. These results may help us understand how we can produce life-saving therapies for patients who need new blood stem cells.</p> <h2>Growing life-saving therapies in the lab</h2> <p>To treat aggressive blood cancers such as leukaemia, patients often need extremely high doses of chemotherapy; a <a href="https://www.cancer.nsw.gov.au/myeloma/diagnosis-and-treatment/treatment/types-of-treatment/stem-cell-transplant#:%7E:text=A%20stem%20cell%20transplant%20involves%20killing%20blood%20cells,they%20are%20collected%20beforehand%20and%20kept%20in%20storage." target="_blank" rel="noopener">blood stem cell transplant</a> then regenerates blood after the treatment. These are life-saving therapies but are restricted to patients who have a suitable tissue-matched donor of blood stem cells.</p> <p>A way around this problem would be to grow more blood stem cells in the lab. Unfortunately, past experiments have shown that harvested adult blood stem cells lose their transplantation potential if grown in the lab.</p> <p>The discovery of <a href="https://en.wikipedia.org/wiki/Induced_pluripotent_stem_cell" target="_blank" rel="noopener">induced pluripotent stem cells</a> – stem cells made out of adult cells – in 2006 led to a promising new approach. Induced pluripotent stem cells are made from the patient’s own cells, so there is no problem with tissue rejection, or the ethical issues surrounding the use of IVF embryos.</p> <p>These cell lines are similar to embryonic stem cells, so they have the potential to form any tissue or cell type – hence, they are “pluripotent”. In theory, pluripotent stem cell lines could provide an unlimited supply of cells for blood regeneration because <a href="https://en.wikipedia.org/wiki/Immortalised_cell_line" target="_blank" rel="noopener">they are immortalised</a> – they can grow in the lab indefinitely.</p> <p>But the development of processes to allow us to grow particular types of tissues, organs and cell types – such as blood – has been slow and will take decades to advance. One must mimic the complex process of embryogenesis in the dish!</p> <h2>Engineering an embryonic heart</h2> <p>Current understanding of how embryonic blood stem cells develop is based on animal models. Experiments with anaesthetised zebrafish embryos have shown that blood stem cells arise in the wall of <a href="https://pubmed.ncbi.nlm.nih.gov/20154733/" target="_blank" rel="noopener">the main blood vessel, the aorta</a>, shortly after the first heartbeat. For ethical reasons, it’s obvious this type of study is not possible in human embryos.</p> <p>This is why we wanted to engineer an embryonic heart model in the lab. To achieve this, we used <a href="https://www.elveflow.com/microfluidic-reviews/general-microfluidics/a-general-overview-of-microfluidics/" target="_blank" rel="noopener">microfluidics</a> – an approach that involves manipulating extremely small volumes of liquids.</p> <p>The first step in generating blood stem cells from pluripotent stem cells is to coax the latter to form the site where blood stem cells start growing. This is known as the AGM region (aorta-gonad-mesonephros) of the embryo.</p> <p>Our miniature heart pump and circulation (3 by 3 centimetres) mimics the mechanical environment in which blood stem cells form in the human embryo. The device pumps culture media – liquids used to grow cells – around a microfluidic circuit to copy what the embryo heart does.</p> <h2>A step closer to treatment</h2> <p>Once we got the cells to form the AGM region by stimulating cells on day two of starting our cell culture, we applied what’s known as pulsatile circulatory flow from day 10 to day 26. Blood precursors entered the artificial circulation from blood vessels lining the microfluidic channels.</p> <p>Then, we harvested the circulating cells and grew them in culture, showing that they developed into various blood components – white blood cells, red blood cells, platelets, and others. In-depth analysis of gene expression in single cells showed that circulatory flow generated aortic and blood stem precursor cells found in the AGM of human embryos.</p> <p>This means our study has shown how pulsatile circulatory flow enhances the formation of blood stem cell precursors from pluripotent stem cells. It’s knowledge we can use in the future.</p> <p>The next step in our research is to scale up the production of blood stem cell precursors, and to test their transplant potential in immune-deficient mice that can accept human transplants. We can do this by using large numbers of pluripotent stem cells grown in bioreactors that also mechanically stimulate blood stem cell formation.</p> <p>If we can easily produce blood stem cells from pluripotent stem cell lines, it would provide a plentiful supply of these cells to help treatments of cancer or genetic blood diseases.</p> <p><strong>This article originally appeared on <a href="https://theconversation.com/scientists-have-mimicked-an-embryos-heart-to-unlock-the-secrets-of-how-blood-cells-are-born-190530" target="_blank" rel="noopener">The Conversation</a>.</strong></p> <p><em>Image: UNSW</em></p>

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“Is this a joke?”: Male finalist in Women in Technology award sparks debate

<p>A list of finalists for an award recognising women in technology and science has sparked controversy due to the inclusion of a male executive.</p> <p>Simon Button, the Group Chief Technology Officer at radiology specialist group Qscan, was announced as one of four finalists for the new 2022 Inspiring Diversity in STEM Award because he “inspires diversity” and empowers women, according to the organisation.</p> <p>The annual awards are run by Women In Technology (WiT), Queensland’s peak industry body for women in technology and life sciences, and aim to “recognise outstanding achievement and give women the recognition they deserve”.</p> <p>WiT Chair Iyari Cevallos said this year’s awards were themed to be a tribute to the contribution of women in defining, shaping and growing the digital economy.</p> <p>“As we celebrate and reflect on 25 years of Women in Technology it is as important to focus on the future,” Ms Cevallos said.</p> <p>“I believe we have the ability and responsibility to dream big, to visualise achievements for the women still to come, to continue to increase our energy and momentum in leading and motivating current and future generations of women.</p> <p>“We've created an opportunity to rally around our outstanding talent, unlock their potential, promote each other and ourselves - impacting our community in a way that creates positivity beyond the event itself.”</p> <p>Mr Button made the finalist list along with Professor Amy Mullens, a psychologist with an interest in marginalised communities, pharmaceutical researcher Dr Jyoti Sharma, and Professor Kym Rae, a physically disabled Research Fellow in Indigenous health.</p> <p>The new award has been introduced to celebrate the “ongoing commitment and tireless efforts of all leaders regardless of gender, age or background”, but some have taken to social media to share their disapproval of the move.</p> <p>“Lol is this a joke,” one woman commented.</p> <p>“That’s a long way to say you reward men for doing the bare minimum,” another said.</p> <p>“Having a male executive is certainly showing someone who ‘leads by example’. It’s just the most common example that already exists in STEM,” a third added.</p> <p>“He sure must’ve been the best pick to be a finalist for someone who ‘inspires diversity’ and creates a ‘sense of belonging’ for WiT out of all the nominees.</p> <p>“I mean, how else would you explain an executive up there with two professors and a doctor?”</p> <p>In a <a href="https://www.facebook.com/WiTqld/posts/pfbid02ajHaMedtDTKmRkWpiKHhqB4sTvEyEYb7w54zckFGcMPmNtmEBGETrthcadKxwKMwl" target="_blank" rel="noopener">Facebook post</a> announcing the finalists, WiT described Mr Button as a “digital leader” who “champions diversity and equity in the organisations he leads”, which includes the not-for-profit Hummingbird House, Queensland’s only children’s hospice that supports kids with life-limiting illnesses.</p> <p>“He thrives to create teams with high levels of diversity to drive increased creativity and higher orders of innovation,” the post read.</p> <p>“Nothing gives Simon greater satisfaction than leading teams by giving people the time and space to develop, learn and deliver outstanding outcomes under his stewardship.</p> <p>“He believes that one of the most important responsibilities modern, contemporary digital leaders have is to lead, mentor and shape tomorrow’s technology and business leaders.”</p> <p> </p> <p><em>Image: WiT: Women In Technology (Facebook)</em></p>

Technology

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Children’s stories can challenge stereotypes

<p>As book week draws near, new research shows the potential power of children’s books to challenge gendered stereotypes in science, technology, engineering and maths. Meanwhile Australian children are spoilt for choice when it comes to diverse books, keep reading below for some recommendations.</p> <p>A research team from the Netherlands, the United States and Canada found when children were read a story that countered stereotypes, for example about a girl who’s good at maths, afterwards they were less likely to hold gendered stereotypes than the control group. The paper is published in PLOS one.</p> <p>The study involved reading aloud to more than 300 North American six to eleven-year-olds, with each child randomly assigned to be read one of three different books. </p> <p>The first story was stereotype-consistent, in which a boy character performed well in maths, while a girl was shown liking and excelling in reading. The second was a counter-stereotypical case, where the story was the same but the characters’ genders were reversed. The third was a neutral story where swimming and tennis replaced the activities of maths and reading.</p> <p>After being read one of the three, the children completed a child-friendly ‘implicit association test’, which involved sorting maths words (like addition, count, math, numbers) and reading related words (like books, letters, words, read) into boy and girl categories. They also completed a ‘self-concept’ test, answering questions like ‘how much do you like maths?’</p> <p>While gendered stereotypes about STEM can be formed early, an important finding of the study was that children’s attitudes are malleable, and books which counter stereotypes can help challenge the status quo.</p> <p>The findings are significant given globally and in Australia, women and girls are under-represented in STEM fields and professions, with gendered perceptions a contributing factor, acting as a barrier to girls’ engagement. </p> <p>Jo Panckridge is a teacher-librarian and the Victorian president of the Children’s Book Council of Australia. She says diversity in all its forms is having a huge impact in the publishing world. </p> <p>For Australian books offering an antidote to traditional gendered views about STEM, Panckridge reels off a long list of titles, starting with Our Little Inventor by Sher Rill Ng. It’s about a girl who invents an air purifying device to solve pollution.</p> <p>Everyday Wonders by Natala Graetz, is brilliant for younger readers Panckridge says. The book highlights mothers who are “ordinary but powerful women – they can be doctors, they can be lawyers, they can be scientists, they can be all sorts of things”.</p> <p>Stellarphant by James Foley tells the story of a girl elephant who wants to be an astronaut but is refused by the managers at Space Command. The picture book showcases “a great example of persistence and resilience” and the love of engineering, space and mathematics, Panckridge says.</p> <p>Heroes, Rebels and Innovators by Karen Wyld celebrates the significant contributions of seven iconic Aboriginal and Torres Strait Islander people and is shortlisted for the CBCA’s Eve Pownall Award for factual books.</p> <p>Andrea Beaty’s Ada Twist, Scientist and Rosie Revere, Engineer deserve a mention, along with Alex Miles’ series Girl Geeks about a group of ten-year-olds who like to code and play video games. </p> <p>“Dreaming with eyes open…” is the theme for this year’s CBCA book week (August 20 – 26). </p> <p>Panckridge says it is both a celebration of First Nations’ storytellers, as well as more broadly “an invitation for children, and teens and young adults, to dream to venture into books, to lose yourself in books and explore the places and people and experiences that stories of this country enable us to understand and to learn from.”</p> <p><strong>This article originally appeared on <a href="https://cosmosmagazine.com/people/social-sciences/childrens-books-combat-stereotypes/" target="_blank" rel="noopener">cosmosmagazine.com</a> and was written by Petra Stock.</strong></p> <p><em>Image: Shutterstock</em></p>

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“Benjamin Button” mice could pave way for reverse ageing

<p>If the three blind mice from the iconic nursery rhyme were living in molecular biologist Dr David Sinclair’s lab at Harvard Medical School, they might not be blind for very long.</p> <p>Dr Sinclair and his team at Harvard Medical School have been using proteins that can turn adult cells into stem cells - a kind of cell that can be turned into any of the specialised cells our bodies need.</p> <p>These stem cells have been helping restore the sight of old mice with damaged retinas, essentially making them younger versions of themselves.</p> <p>“It’s a permanent reset, as far as we can tell, and we think it may be a universal process that could be applied across the body to reset our age,” Dr Sinclair said about his research, which was published in late 2020.</p> <p>The Australian scientist has spent the past 20 years studying ways to reverse the effects of ageing - including the diseases that can afflict us as we get older.</p> <p>“If we reverse ageing, these diseases should not happen,” he said.</p> <p>During a health and wellness talk at Life Itself, Dr Sinclair said the technology is available and it’s only a matter of when we decide to use it.</p> <p>“We have the technology today to be able to go into your hundreds without worrying about getting cancer in your 70s, heart disease in your 80s and Alzheimer’s in your 90s,” he said.</p> <p>“This is the world that is coming. It’s literally a question of when and for most of us, it’s going to happen in our lifetime.”</p> <p>Whitney Casey, an investor who has partnered with Dr Sinclair to create a DIY biological age test, said the researcher wants to “make ageing a disease”.</p> <p>“His research shows you can change ageing to make lives younger for longer,” she said.</p> <p>Dr Sinclair said that when it comes to how modern medicine addresses sickness, it doesn’t tackle the underlying cause, which is usually “ageing itself”.</p> <p>“We know that when we reverse the age of an organ like the brain in a mouse, the diseases of ageing then go away. Memory comes back, there is no more dementia,” he continued.</p> <p>“I believe that in the future, delaying and reversing ageing will be the best way to treat the diseases that plague most of us.”</p> <p>Dr Sinclair’s research comes amid a global effort by scientists working to reprogram adult cells into stem cells, started by Japanese researcher Shinya Yamanaka, who won a Nobel Prize for reprogramming adult skin cells into behaving like embryonic (or pluripotent) stem cells.</p> <p>These “induced pluripotent stem cells” became known as “Yamanaka factors”, with later research finding that exposing cells to four of the main Yamanaka factors could remove signs of ageing.</p> <p>Since their original study, where they discovered that damaged cells were able to be rejuvenated by injecting three of these factors into the eyes of mice, Dr Sinclair and his lab have reversed ageing in mouse brains and muscles, and are now working on a mouse’s whole body.</p> <p>Dr Sinclair said their discovery indicated that there is a “back-up copy” of youthful information stored in the body, which he calls the “information theory of ageing”.</p> <p>“It’s a loss of information that drives ageing cells to forget how to function, to forget what type of cell they are,” he revealed.</p> <p>“And now we can tap into a reset switch that restores the cell’s ability to read the genome correctly again, as if it was young.”</p> <p><em><span id="docs-internal-guid-5569962c-7fff-455b-2538-0661dd2d0f60">Image: Getty Images</span></em></p>

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Stems cells could be used to treat macular degeneration

<p>A study at Cedars-Sinai Medical Center in Los Angeles has found that an injection of stem cells in the eyes could be an effective treatment for vision loss caused by age-related macular degeneration. Currently, there is no treatment that slows the progression of the disease, which is the leading cause of vision loss in people over 65.</p> <p>The study’s lead author, Shaomei Wang, MD, PhD explained, “This is the first study to show preservation of vision after a single injection of adult-derived human cells into a rat model with age-related macular degeneration.” Published in the journal STEM CELLS, the study resulted in 130 days of preserved vision in laboratory rat; this equates roughly to 16 years for a human.</p> <p>When animals with macular degeneration were injected with stem cells created using adult skin cells, healthy cells began to migrate around the retina, forming a protective layer. This shield prevented ongoing degeneration of the vital retinal cells responsible for vision.</p> <p>For those who don’t know, age-related macular degeneration occurs when the small central portion of the retina – the macula – deteriorates. Aside from age, other causes of macular degeneration include a genetic predisposition, and environmental factors.</p> <p>The next steps in the process include testing the safety of the stem cell injection in preclinical animal studies. In the future, clinical trials will be designed to test any potential benefit in patients with later-stage age-related macular degeneration.</p> <p><span style="text-decoration: underline;"><strong><a href="http://www.sciencedaily.com/releases/2015/04/150414093554.htm" target="_blank">Source: Science Daily</a></strong></span></p>

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